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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha : |
15/05/2015 |
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Actualizado : |
03/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
VIÑOLES, C.; FORSBERG, M.; BANCHERO, G.; RUBIANES, E. |
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Afiliación : |
CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Centre for Reproductive Biology, Department of Clinical Chemistry, Swedish University of Agricultural Sciences, Uppsala, Swed;Universidad de la República (UdelaR)/ Facultad de Veterinaria; M. FORSBERG, Centre for Reproductive Biology, Department of Clinical Chemistry, Swedish University of Agricultural Sciences, Uppsala, Swed; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. RUBIANES, Universidad de la República (UdelaR)/ Facultad de Veterinaria. |
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Título : |
Ovarian follicular dynamics and endocrine profiles in Polwarth ewes with high and low body condition. |
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Fecha de publicación : |
2002 |
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Fuente / Imprenta : |
Animal Science, 2002, v. 74, no. 3, p. 539-545. |
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DOI : |
10.1017/S1357729800052693 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 20 July 2001 // Accepted 14 November 2001 // Published online by Cambridge University Press: 18 August 2016. |
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Contenido : |
In this investigation we tested the hypothesis that static body condition (BC) of the ewe affects oestradiol and FSH with implications for subsequent follicular growth and turn-over. Sixteen Polwarth ewes were selected from a flock according to their BC score (scale: 1 emaciated; 5 obese). High BC (HBC) ewes (no. = 8) had a BC score of 4.1 (s.e. 0.1) and low BC (LBC) ewes (no. = 8) had a BC score of 1.9 (s.e. 0.1). Daily ultrasound examinations were performed and blood samples for progesterone, oestradiol and follicle-stimulating hormone (FSH) determination were collected. All HBC ewes (8/8) exhibited three waves of follicular development, while four LBC ewes (418) had two waves and the other four (4/8) had three waves of follicular development (P less than or equal to0.05) during the interovulatory period. Overall, the emergences of 33 out of 44 follicular waves were preceded by significant increases in FSH concentrations. Maximum FSH concentrations were detected 0.9 +/- 0.2 days before wave emergence. Oestradiol concentrations increased significantly during the growing phase in 38 out of 44 large follicles. A negative correlation between oestradiol and FSH was observed in HBC ewes. A similar inhibitory effect of oestradiol on FSH was observed in LBC ewes, irrespective of whether they developed two or three follicular waves. However, a longer period with high FSH was needed to promote the emergence of the second follicular wave in two-wave LBC ewes. Four HBC ewes had twin ovulations but no LBC ewes did (P less than or equal to 0.05). In HBC ewes, the follicular phase was characterized by lower oestradiol (6.5 (s.e. 1.0) pmol/l) but higher mean FSH concentrations (2.4 (s.e. 0.4) mug/l) than in LBC ewes (8.9 (s.e. 1.2) pmol/l and 2.0 (s.e. 0.3) mug/l, respectively, P less than or equal to 0.05). The present results suggest that BC influences the pattern of follicular dynamics through changes in the endocrine milieu. Higher FSH concentrations during the follicular phase in HBC ewes, which allowed an extended period of follicular recruitment from a significantly larger pool of small antral follicles could explain the higher ovulation rate observed in this group. MenosIn this investigation we tested the hypothesis that static body condition (BC) of the ewe affects oestradiol and FSH with implications for subsequent follicular growth and turn-over. Sixteen Polwarth ewes were selected from a flock according to their BC score (scale: 1 emaciated; 5 obese). High BC (HBC) ewes (no. = 8) had a BC score of 4.1 (s.e. 0.1) and low BC (LBC) ewes (no. = 8) had a BC score of 1.9 (s.e. 0.1). Daily ultrasound examinations were performed and blood samples for progesterone, oestradiol and follicle-stimulating hormone (FSH) determination were collected. All HBC ewes (8/8) exhibited three waves of follicular development, while four LBC ewes (418) had two waves and the other four (4/8) had three waves of follicular development (P less than or equal to0.05) during the interovulatory period. Overall, the emergences of 33 out of 44 follicular waves were preceded by significant increases in FSH concentrations. Maximum FSH concentrations were detected 0.9 +/- 0.2 days before wave emergence. Oestradiol concentrations increased significantly during the growing phase in 38 out of 44 large follicles. A negative correlation between oestradiol and FSH was observed in HBC ewes. A similar inhibitory effect of oestradiol on FSH was observed in LBC ewes, irrespective of whether they developed two or three follicular waves. However, a longer period with high FSH was needed to promote the emergence of the second follicular wave in two-wave LBC ewes. Four HBC ewes had twin o... Presentar Todo |
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Palabras claves : |
CONFORMATION; EWES; FOLLICLES; FSH; OESTRADIOL. |
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Thesagro : |
MEDICINA VETERINARIA. |
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Asunto categoría : |
L53 Fisiología Animal - Reproducción |
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Marc : |
LEADER 03013naa a2200253 a 4500
001 1052635
005 2020-02-03
008 2002 bl uuuu u00u1 u #d
024 7 $a10.1017/S1357729800052693$2DOI
100 1 $aVIÑOLES, C.
245 $aOvarian follicular dynamics and endocrine profiles in Polwarth ewes with high and low body condition.
260 $c2002
500 $aArticle history: Received 20 July 2001 // Accepted 14 November 2001 // Published online by Cambridge University Press: 18 August 2016.
520 $aIn this investigation we tested the hypothesis that static body condition (BC) of the ewe affects oestradiol and FSH with implications for subsequent follicular growth and turn-over. Sixteen Polwarth ewes were selected from a flock according to their BC score (scale: 1 emaciated; 5 obese). High BC (HBC) ewes (no. = 8) had a BC score of 4.1 (s.e. 0.1) and low BC (LBC) ewes (no. = 8) had a BC score of 1.9 (s.e. 0.1). Daily ultrasound examinations were performed and blood samples for progesterone, oestradiol and follicle-stimulating hormone (FSH) determination were collected. All HBC ewes (8/8) exhibited three waves of follicular development, while four LBC ewes (418) had two waves and the other four (4/8) had three waves of follicular development (P less than or equal to0.05) during the interovulatory period. Overall, the emergences of 33 out of 44 follicular waves were preceded by significant increases in FSH concentrations. Maximum FSH concentrations were detected 0.9 +/- 0.2 days before wave emergence. Oestradiol concentrations increased significantly during the growing phase in 38 out of 44 large follicles. A negative correlation between oestradiol and FSH was observed in HBC ewes. A similar inhibitory effect of oestradiol on FSH was observed in LBC ewes, irrespective of whether they developed two or three follicular waves. However, a longer period with high FSH was needed to promote the emergence of the second follicular wave in two-wave LBC ewes. Four HBC ewes had twin ovulations but no LBC ewes did (P less than or equal to 0.05). In HBC ewes, the follicular phase was characterized by lower oestradiol (6.5 (s.e. 1.0) pmol/l) but higher mean FSH concentrations (2.4 (s.e. 0.4) mug/l) than in LBC ewes (8.9 (s.e. 1.2) pmol/l and 2.0 (s.e. 0.3) mug/l, respectively, P less than or equal to 0.05). The present results suggest that BC influences the pattern of follicular dynamics through changes in the endocrine milieu. Higher FSH concentrations during the follicular phase in HBC ewes, which allowed an extended period of follicular recruitment from a significantly larger pool of small antral follicles could explain the higher ovulation rate observed in this group.
650 $aMEDICINA VETERINARIA
653 $aCONFORMATION
653 $aEWES
653 $aFOLLICLES
653 $aFSH
653 $aOESTRADIOL
700 1 $aFORSBERG, M.
700 1 $aBANCHERO, G.
700 1 $aRUBIANES, E.
773 $tAnimal Science, 2002$gv. 74, no. 3, p. 539-545.
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INIA Tacuarembó (TBO) |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
25/04/2018 |
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Actualizado : |
25/04/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GALLINO, J.P.; RUIBAL, C.; CASARETTO, E.; FLEITAS, A.L.; BONNECARRERE, V.; BORSANI, O.; VIDAL, S. |
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Afiliación : |
JUAN P. GALLINO, Universidad de la República (UdelaR)/ Facultad de Ciencias; CECILIA RUIBAL, Universidad de la República (UdelaR)/ Facultad de Ciencias; ESTEBAN CASARETTO, Universidad de la República (UdelaR)/ Facultad de Agronomía; ANDREA L. FLEITAS, Universidad de la República (UdelaR)/ Facultad de Ciencias; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OMAR BORSANI, Universidad de la República (UdelaR)/ Facultad de Agronomía; SABINA VIDAL, Universidad de la República (UdelaR)/ Facultad de Ciencias. |
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Título : |
A dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 2018, v.9, Article number 262. (2 March 2018). OPEN ACCESS |
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DOI : |
10.3389/fpls.2018.00262 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018. |
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Contenido : |
ABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress.
© 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal. MenosABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (Gm... Presentar Todo |
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Palabras claves : |
ABIOTIC STRESS; ARABIDOPSIS; DROUGHT; EIFiso4G; SOYBEAN CROP; TRANSLATION INITIATION. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9385/1/Frontiers-in-Plant-Science.-2018.fpls-09-00262.pdf
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Marc : |
LEADER 03453naa a2200289 a 4500
001 1058480
005 2018-04-25
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2018.00262$2DOI
100 1 $aGALLINO, J.P.
245 $aA dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis.$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018.
520 $aABSTRACT. Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress. © 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal.
653 $aABIOTIC STRESS
653 $aARABIDOPSIS
653 $aDROUGHT
653 $aEIFiso4G
653 $aSOYBEAN CROP
653 $aTRANSLATION INITIATION
700 1 $aRUIBAL, C.
700 1 $aCASARETTO, E.
700 1 $aFLEITAS, A.L.
700 1 $aBONNECARRERE, V.
700 1 $aBORSANI, O.
700 1 $aVIDAL, S.
773 $tFrontiers in Plant Science, 2018$gv.9, Article number 262. (2 March 2018). OPEN ACCESS
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